Ultraviolet spectroscopy
(UV): Spectroscopy
using photons
in the 400-100 nm range. Photons
in this energy range cause electronic excitation,
so ultraviolet spectroscopy
is particularly useful to study aromatic,
conjugated,
and other substances containing a pi
bond. Modern ultraviolet spectrophotometers
also cover the visible light range (700-400 nm), in which case the
technique is called ultraviolet-visible spectroscopy
(UV-vis). Ultraviolet and ultraviolet-visible spectrometry
is useful to detect and quantify the presence of substances that
absorb UV and/or visible light.
Ultraviolet absorption spectrum
of lycopene,
showing
significant absorption peaks at 443, 471, 502 nm, with λmax
= 471 nm.