Where:
[α] = the specific rotation in
degrees of specified wavelength of light and specified
temperature
t = temperature of measurement
λ = wavelength of light; usually the D line from a sodium
vapor lamp (589 nm)
α = observed rotation in degrees
l = path length (length of sample tube) in decimeters (one
decimeter = 10 cm)
c = concentration of sample measured in grams per milliliter
of solution