a. What is the purpose of this step?

b. Why and where is sand used to prepare the column?

c. Why is the stationary phase treated with a 1% NEt₃ solution before the sample is applied?

d. How do you find out which part of the eluent contains the desired epoxide?

2. NMR spectroscopy

a. Which solvent is used to acquire the NMR spectra of the final product? Why?

b. What is the "proper" labeling for the NMR tube?

c. Which information can one extract from the ¹H-NMR and the ¹³C-NMR spectrum?

3. GC/MS spectrometry

a. A student acquires the GC/MS spectrum for his epoxide using a modified b-cyclodextrin column. Which conclusions can the student draw from the data below?

time (min)	area (units)
1.25	5000
5.10	300
9.85	550
10.15	200
10.5	50

Note: The last three peaks exhibit the same molecular weight.

b. Outline a brief procedure how to prepare the sample.

c. What is the "proper" procedure to inject the sample into the GC?