1. General

Since the course goes rapidly towards the end, it is time to get organized for the final exam.

1. You should have finished all experimental work by the end of meeting 9 (9/7/2006). If this is not the case, you will aumatically receive a failing grade. You will not be allowed to perform any experimental work during meeting 10 since this meeting is for clean-up, TA evaluation and NMR work.

2. You should review the experiments and the theory behind the techniques used in the lab. How and why are these techniques used? This part should be done before attending the review sessions for the final that will be on Monday, 9/11/2006 from 5-6 pm (part 1) and Tuesday 9/12/2006 from 11-12 pm (part 2). The review session will be held by the instructor and address important topics in regard of the individual experiments. No questions will be answered what will be on the final exam.

2. Experiment

a. TLC part

Whenever you want/need to optimize a solvent for chromatography, you need to ask you the following questions

- What is the polarity of the stationary phase?

- What is the polarity of the mobile phase? How strongly does the compound interact with the stationary phase?

- Which solvent cause the compounds to move to a certain degree? How good is the separation?

- How can the separation be improved by addition of a polar/non-polar solvent?

- How do I prepare the solution that is used to spot the TLC plate?

- What do I use for spotting?

b. NMR simulation

The ACD software enables you to simulate NMR (H and C) spectra for simple compounds up to 50 atoms. The software allows you to expand ranges of interest easily to analyze multiplet structures and coupling. Like most simulation programs, the accuracy is limited because they assume a normal arrangement of groups and often does not correctly account for additional intramolecular bonds. In addition, the solvent effect is not accounted for either in those simulations.