S-Adenosyl-L-methionine:Mg-protoporphyrin IX methyltransferase (ChlM) in C. reinhardtii

S-Adenosyl-L-methionine:Mg-protoporphyrin IX methyltransferase catalyzes the methylation of Mg-protoporphyrin in the following reaction:

View the chlorophyll biosynthesis pathway

The reaction is thought to prevent the spontaneous decarboxylation of the 6-propionate during later steps in chlorophyll biosynthesis. BchM has been identified as encoding the methyl transferase in bacteria and homologs have been found in algae and plants. The following is a contigous cDNA sequence with its translation below for chlM homolog in Chlamydomonas reinhardtii.

-282                    GCACGAGGtTAAAAATGCAGTGCCTAAACACCAAGCCTTTTA
-240  CCTCGGCCCGCCCGGCTGTGGCCCGGCGGAGCGTCGTCGCGCATGCCTCGCGCCCGGCCC
-180  AGGCTGCCGCGCTGGCCTCTGCCATGACCGTGGCGACCACCTCCGCTGCTATGGCTTcCT
-120  GAGATTGCTCAAACTGCCGATGTTGGCTCGCTGACCTTCGCGGTTGGCGGAGTGGGCGCT
-60   GTTGTGGGCCTGGGCGCCCTGCTTGTCGCTACTGACCCTCAGAAGCGCCGCTCGGAGCAG
+1    ATGAAGTCATTCGACGGCGATGAGAAGGAGGCGGTGAAGGACTACTTCAACACTGCCGGC
      M  K  S  F  D  G  D  E  K  E  A  V  K  D  Y  F  N  T  A  G
+61   TTCGAGCGCTGGCGCAAGATCTACGGCGAGACGGACGAGGTGAACAAGGTGCAGCTGGAC
      F  E  R  W  R  K  I  Y  G  E  T  D  E  V  N  K  V  Q  L  D
+121  ATCCGCACTGGCCACGCGCAGACGGTGGACAAGGTGCTGCGCTGGGTGGACGAGGAGGGC
      I  R  T  G  H  A  Q  T  V  D  K  V  L  R  W  V  D  E  E  G
+181  AGCGTGCAGGGCATCACCGTGGCGGACTGCGGCTGCGGGACAGGCAGCCTGGCCATCCCG
      S  V  Q  G  I  T  V  A  D  C  G  C  G  T  G  S  L  A  I  P
+241  CTGGCGCTGCGCGGCGCCGCCGTCAGCGCCTCCGACATCTCCGCCGCCATGGCGTCTGAG
      L  A  L  R  G  A  A  V  S  A  S  D  I  S  A  A  M  A  S  E
+301  GCGGAGCAGCGCTACCAGCAGGCGGTGGCGGCCGGGCAGGGCAAGGCGCCCAAGGTGGCG
      A  E  Q  R  Y  Q  Q  A  V  A  A  G  Q  G  K  A  P  K  V  A
+361  CCCAAGTTCGAGGCGCTGGACCTGGAGTCCGTCAAGGGCAAGTACGACACGGTCACTTGC
      P  K  F  E  A  L  D  L  E  S  V  K  G  K  Y  D  T  V  T  C
+421  CTGGACGTGATGATTCACTACCCCCAGGACAAGGTGGACGCCATGATCACGCACCTGGCG
      L  D  V  M  I  H  Y  P  Q  D  K  V  D  A  M  I  T  H  L  A
+481  GGTCTGTCCGACCGCCGCCTCATCATcTCGTTCGCACCCAAGACGCTGTCGTACAGCATC
      G  L  S  D  R  R  L  I  I  S  F  A  P  K  T  L  S  Y  S  I
+541  CTCAAGCGCATTGGCGAGCTGTTCCCCGGCCCCAGCAAGGCCACCCGCGCCTACCTGCAC
      L  K  R  I  G  E  L  F  P  G  P  S  K  A  T  R  A  Y  L  H
+601  CGTGAGGAGGACGTGGAGGCTGCCCTCAAGCGCGCCGGCTTCAAGGTCACCAAGCGTGAG
      R  E  E  D  V  E  A  A  L  K  R  A  G  F  K  V  T  K  R  E
+661  ATGACCgCCaCCAGCTTTTACTTcTcGCGCCTGCTGGAGGCCATCCgCGAGTAATcTcGC
      M  T  A  T  S  F  Y  F  S  R  L  L  E  A  I  R  E  *
+721  AAACGTTtTcGTGTAAATAGCGTAgCAGTGCAGGACTGGTGTAAGGACTgATGGACTCGG
+781  ACCGGCgCTgCGGCCGGGTATgAGGGCATGGGCGTGGGCgCTGCTAGGGTGCTACGGTGC
+841  AGCGCGGTTTTgACGGGCTATAgACATTTATGTGACTGAGTGACGGGCGGATAgCCAAAA
+901  CGATTGTGTGAACGACTATAgACAGACTGACTTCAAgGGATgAGGATGGGCAAgGGGGGA
+961  CCCTCCaACgCCgATGGAgAgAGTGGTAAgCGAGCTAgATgTgAGTgAGGAAAAGGTGGA
+1021 GGTGGACGCTCAAgCCaCGCgCTGTGGAGTGCaCaCGGGACGCAAGGTGCaCCGGTGCaC
+1081 GAgCCgCCAACTGACTGTAAAATTgAAGGCCGGAAAAAA